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. 2018 Apr 4;8:5660. doi: 10.1038/s41598-018-23897-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence images of C. pneumoniae K6, H15-1957-3C and H15-1957-10C at 32 and 48 hours post infection (hpi). Shown are representative immunofluorescence images illustrating the morphology of C. pneumoniae K6 (a–h), H15-1957-3C (i–p), H15-1957-10C (q–x) at 32 hpi (columns 1 and 3) and 48 hpi (columns 2 and 4) in the presence (columns 1–2) or absence (columns 3–4) of cycloheximide. Strains were grown at 37 °C (top line per strain; lines 1, 3, 5) and 28 °C (bottom lane per strain; lines 2, 4, 6). The size bar indicates 5 µm. Chlamydial inclusions are shown in green, the LLC-MK2 nuclei (DAPI) are shown in blue.