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. 2018 Apr 4;8:5660. doi: 10.1038/s41598-018-23897-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The average inclusion size of C. pneumoniae K6, H15-1957-3C and H15-1957-10C is significantly decreased following incubation at 28 °C compared to 37 °C. Shown is a boxplot comparing the inclusion size (µm²) distribution for strains a) C. pneumoniae K6, H15-1957-3C and H15-1957-10C, and b) S15-834C and S15-834K following 48 h of incubation at 28 °C (dark grey) or 37 °C (light grey). Filled circles represent outliers (>1.5x interquartile range), while asterisks represent extreme values (>3x interquartile range). The inclusion size was determined using a Leica DMLB fluorescence microscope (Leica Microsystems, Wetzlar, Germany) and a UI-2250SEC-HQ camera (uEye, IDS Imaging Development Systems GmbH, Obersulm, Germany) and analysed with the BonTec measuring and archiving software (BonTec, Bonn, Germany). Boxplots were created by the SPSS Statistics software. An unpaired t test was used for statistical analysis, and a p-value of less than 0.05 was considered significant.