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. 2018 Apr 4;8:5660. doi: 10.1038/s41598-018-23897-z

Figure 4.

Figure 4

Titration by sub-passage confirmed that the infectivity of C. pneumoniae K6, H15-1957-3C and H15-1957-10C is significantly higher at 37 °C while the infectivity of strains S15-834C and S15-834K is increased at 28 °C. LLC-MK2 cells were infected with one chlamydial strain of a) either C. pneumoniae K6, H15-1957-3C, H15-1957-10C, or b) S15-834C, S15-834K and incubated for 48 hours at 28 °C or 37 °C in the presence (white bars) or absence (black bars) of cycloheximide. At 48 hours post infection, monolayers were scraped into the supernatant and used for titration by sub-passage. Inclusion forming units (IFU/ml) are shown in a logarithmic scale. Asterisks indicate statistical significance (p < 0.05) by unpaired t test.