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. 2018 Apr 4;8:5660. doi: 10.1038/s41598-018-23897-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The ultrastructure of C. pneumoniae K6, H15-1957-3C and H15-1957-10C is similar with fully developed inclusions at 37 °C as opposed to 28 °C, while strains S15-834C and S15-834K form mature inclusions at 28 °C and show indication for the presence of aberrant bodies (ABs) at 37 °C. Cultures were fixed in glutaraldehyde at 48 hours post infection and processed for transmission electron microscopy. Shown is the ultrastructure of C. pneumoniae K6, H15-1957-3C, H15-1957-10C, S15-834C and S15-834K (top to bottom) following incubation at 37 °C (left column) or 28 °C (right column). The size bar indicates 10 µm. N = nucleus, Nc = nucleolus, Chlc = chlamydial inclusion, Cy = cytoplasm, I = intercellular space.