Table 1.

Details of snakes positive by real-time PCR for Chlamydiaceae. Samples were collected from asymptomatic alive snakes or taken at necropsy from dead snakes.

CollectionID	Snake no.	Sample no.1/Strain ID	Snake species	Live (L) Necropsy (N)	Real-time PCR (∅ Ct value)	Real-time PCR (∅ quantity, copies/µl)	Arraymate	Isolation
A	1	H15-1957-3C	Pantherophis guttatus	L	24.9	42′511	C. pneumoniae	Yes
	2	H15-1957-10C H15-1957-10K	Atheris squamigera	L	32.5 33.4	330 188	C. pneumonia C. pneumoniae	Yes No
B	3	H15-1971-20C H15-1971-20K	Sanzinia madagascariensis voluntar	L	26.0 30.4	21′767 1′280	Chlamydia4 Chlamydia4	No Not done
	4	H15-1971-22C H15-1971-22K	Python regius	L	29.32 29.7	3′4102 2′105	C. pneumonia C. pneumoniae	No No
C	5	H15-2055-65K	Python m. molurus	L	18.4/UT3	2′354′763/UT3	C. pneumoniae	Not done
	6	H15-2055-69C	Crotalus basilicus	L	29.5	3′182	C. pneumoniae	Not done
	7	H15-2055-70C	Crotalus basilicus	L	26.9	15′388	C. pneumoniae	Not done
	8	H15-2055-71C	Crotalus basilicus	L	25.4	36′997	C. pneumoniae4	Not done
	9	H15-2055-72C	Crotalus basilicus	L	29.2	4′206	C. pneumonia C. muridarum	Not done
	10	H15-2055-79C H15-2055-79K	Python regius	L	33.7 26.2	190 26′165	C. pneumoniae4 C. pneumoniae	Not done Not done
D	11	H15-2833-20C	Python regius	L	29.5	2′547	C. pneumoniae4	Not done
E	12	H15-2898-1C	Eunectes murinus	L	30.9	932	Chlamydia4	No
	13	H15-2898-2C	Eunectes murinus	L	26.5	16′134	C. pneumoniae4	No
	14	H15-2898-3K	Eunectes murinus	L	14.1	212′659′086	Negative5	No
	15	H15-2898-13C	Eunectes murinus	L	29.6	9′245	Chlamydia4	No
F	16	H15-2641-14K	Boa constrictor	L	26.8	17′286	C. abortus	No
G	17	S15-212-K	Crotalus cerastes	N	24.8	35′424	C. pneumoniae4	Not done
H	18	S15-834C S15-834K	Pantherophis guttatus	N	25.0 18.2	14′272 4′372′828	C. caviae C. pneumoniae	Yes Yes

¹C = swab samples from choana; K = swab sample from cloaca.

²Samples diluted 1:10 for analysis.

³UT = undetermined.

⁴Suspected genus/species based on Arraymate probes.

⁵Possible false-positive real-time PCR result.