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. 2018 Apr 4;8:5646. doi: 10.1038/s41598-018-24017-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Seahorse XF96 respirometry analysis of aged mice at day 7 post injury. (a) Respiration profiles of liver mitochondria from control mice (grey), burned mice (black) and burned mice with metformin treatment (100 mg/kg; green). (b) Production of reactive oxygen species as measured by oxidation of DCFDA in isolated mitochondria given substrate (5 mM pyruvate, 3 mM malate, 4 mM ADP); λ = 495 nm, λ’ = 529 nm. Basal (c), state 3 (d) and state 3 u (e) respiration parameters in isolated mitochondria as measured via Seahorse XF96 extracellular flux assays. : control (n = 5); ■: burn (n = 5); : burn + metformin treatment (n = 6). Values are presented as mean ± standard deviation. *p ≤ 0.05; **p ≤ 0.01.

Inline graphic — Seahorse XF96 respirometry analysis of aged mice at day 7 post injury. (a) Respiration profiles of liver mitochondria from control mice (grey), burned mice (black) and burned mice with metformin treatment (100 mg/kg; green). (b) Production of reactive oxygen species as measured by oxidation of DCFDA in isolated mitochondria given substrate (5 mM pyruvate, 3 mM malate, 4 mM ADP); λ = 495 nm, λ’ = 529 nm. Basal (c), state 3 (d) and state 3 u (e) respiration parameters in isolated mitochondria as measured via Seahorse XF96 extracellular flux assays. : control (n = 5); ■: burn (n = 5); : burn + metformin treatment (n = 6). Values are presented as mean ± standard deviation. *p ≤ 0.05; **p ≤ 0.01.