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. 2018 Apr 4;8:5616. doi: 10.1038/s41598-018-23589-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The number of 25-mers in the final k-mer lists used in the NIPT analysis (black) and the maximum number of possible k-mers (white) for each chromosome. The final k-mer lists for autosomes ranged from 7,781,826 (chr 21) to 54,629,103 (chr 2) unique 25-mers. The lists for chromosomes X and Y were the smallest: 5,860,559 and 5,071,089 unique 25-mers, respectively.