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. 2018 Mar 29;9:619. doi: 10.3389/fimmu.2018.00619

Figure 4.

Figure 4

Treatment with the anti-transforming growth factor (TGF)β IgG antibody decreases nephrocalcinosis-related macrophage infiltrates. C57BL/6N mice were either fed a control diet, a high-oxalate diet only or combined with IgG1 or anti-TGFβ antibody treatment for 14 days. (A,B) Flow cytometric analysis of infiltrating CD45+ leukocytes and gating strategy of CD45+ F4/80+ CD11b+ macrophages in kidneys with absolute numbers. (C) Renal infiltrating macrophages identified as pro-inflammatory (M1-like) (CD45+ F4/80+ CD11b+ CX3CR1+ CD206−) macrophages with absolute cell numbers. (D) mRNA expression of inducible nitric oxide synthase (iNOS) was performed on kidney RNA isolates. (E,F) Absolute cell numbers of pro-fibrotic (M2a-like) macrophages (CD45+ F4/80+ CD11b+ CX3CR1+ CD206+ TGFβ+) (E) and anti-inflammatory (M2c-like) macrophages (CD45+ F4/80+ CD11b+ CX3CR1+ CD206+ TGFβ−) (F) in the kidneys. Data are mean ± SEM from six to seven mice per group out of two independent experiments. ns, not significant. *p < 0.05, **p < 0.01, ***p < 0.001 are considered significant.