Figure 2.

Impact of hyperproteinemia on development and survival. Index of development and survival expressed in terms of the cumulative mortality, pupation rate, semi-pupa rate, and larval rate in the (A) negative control (CK) group (n = 92 individuals) and (B,C) the animal model (AM) of hyperproteinemia group (n = 90 individuals). (B) At 24 h after the wandering stage, the spinneret was covered with paraffin wax after spinning silk for 17 h, and approximately 50% of the silk protein remained in the silk gland (SG), while (C) at the wandering stage, the spinneret was completely impermeable and all the silk proteins remained in the SG. The larval spinnerets of B. mori in the CK group were not covered, and approximately 8% of the silk protein remained in the SG. (D) Morphological comparisons of silkworm development between the AM of hyperproteinemia and the CK group. Bars are 1 cm in (D). To observe the morphology of the silkworm, the cocoon shells in the CK group were peeled off every 12 until 48 h after spinning. This treatment also ensured the consistency of the micro-environment among the silkworms that spun their cocoons in the CK group and those that did not in the AM group.