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. 2018 Mar 29;9:302. doi: 10.3389/fphys.2018.00302

Figure 7.

Figure 7

Localization of caspase-3 in fat body (FB) cross-sections at the pre-pupa stage. Bombyx mori larvae were treated and sampled as described in the Figure 1. CK and AM indicate the control and animal model groups, respectively. Cross-sections of the FB were dissected from silkworms at 24, 48, 72, 96, and 120 h after hyperproteinemia was induced. Caspase-3 (green fluorescence) was stained by an anti-caspase-3 antibody conjugated with fluorescein isothiocyanate (FITC). Merge, merged images for FITC and genomic DNA labeled with 4′,6-diamidino-2-phenylindole (DAPI). The red arrows represent the nucleus positions of fat body cells. The bar = 20 μm.