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. 2018 Mar 29;9:302. doi: 10.3389/fphys.2018.00302

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Copyright © 2018 Chen, Wang, Wang, Li, Ma, Wang, Sima and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in the oxidative stress level of the fat body (FB) during metamorphosis. Bombyx mori larvae were treated and sampled as described in the Figure 1. (A) Reactive oxygen species (ROS) staining of the FB. (B) Activities of super oxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) at 24, 48, 72, and 96 h after the induction of hyperproteinemia. Each FB sample, which consisted of a mixture from 10 silkworms, was obtained in triplicate and used for repeat measurement of SOD, CAT, and GST activity levels. ^* and ^** indicate significant differences between the two groups at P < 0.05 and P < 0.01, respectively (n = 3). The bar = 50 μm.