Table 2. Potential mutations contributing to polymyxin resistance and acquired resistance genes.

*Multilocus sequence type as identified through MultiLocus Sequence Typing Server 1.8.

†Variations detected in mgrB, phoPQ and pmrAB potentially causing polymyxin resistance. Significant non-synonymous changes determined by PROVEAN analysis. WT (wild-type) alleles in comparison to 20_GR_12. Displayed as gene impacted, initial amino acid, position and new amino acid. If a dash (–) is shown in front of the position, variant is encoded upstream and if a dash (–) is only displayed, no significant non-synonymous changes were detected in these loci. Insertion sequences (underlined) classified as Δ, identity as per ISFinder and orientation in superscript. Orientation determined as forward, ^F, if transposase is in the same direction as mgrB and conversely, reverse, ^R, if in the opposite direction to mgrB.

‡Acquired antibiotic resistance genes detected via ResFinder 3.0. Classes of antibiotics impacted are displayed as: A, aminoglycoside; B, beta-lactam; F; fosfomycin; M, macrolide; P, phenicol; Q, quinolone; R, rifampicin; S, sulphonamide; T, tetracycline; Tr, trimethoprim. Shading indicates detection of a gene (≥90 % homology, ≥60 % sequence length).