FIG 1 .

Host transcriptomic responses 6 h postinfection are largely MYR1-dependent. Host RNA expression levels in mock-infected HFFs were compared to expression levels of HFFs infected with wild-type RH, RHΔmyr1, and RHΔmyr1::MYR1 tachyzoites. (A) Heat maps displaying expression levels for the 1,915 genes that in the RH-WT-infected cells exhibited a statistically significant increase (q value of <0.05, 10% FDR) of ≥1.5-fold over the expression in mock-infected HFFs. (B) As for panel A, except expression levels are for the 1,370 genes that exhibited a statistically significant decrease of ≥1.5-fold relative to mock infection. Gene names and average RPKMs can be found in Table S1. Seven replicates are shown for each of the four conditions (uninfected and three parasite-infected lines) with the rows 1 to 3, 4/5, and 6/7 being technical replicates of the first, second and third biological experiments, respectively. The log₂ values for all 28 data points for each gene are colored red for the highest value and blue for the lowest value, regardless of the range of values they represent. All heat maps display genes sorted from left to right on the basis of decreasing magnitude of the average difference in expression between cells infected with RH-WT versus RHΔmyr1.