TABLE 1 .
Summary of total RNA-Seq reads mapped
| Infection and sample no. | No. (%) of reads mappeda |
|
|---|---|---|
| Human genome | Toxoplasma genome | |
| Mock | ||
| 1 | 28,303,437 (99.5) | 136,537 (0.5) |
| 2 | 26,656,452 (99.4) | 153,263 (0.6) |
| 3 | 13,637,605 (99.2) | 105,372 (0.8) |
| 4 | 13,007,385 (99.3) | 98,286 (0.7) |
| 5 | 5,280,417 (99.2) | 40,551 (0.8) |
| 6 | 15,675,192 (99.5) | 85,248 (0.5) |
| 7 | 10,225,078 (99.3) | 68,095 (0.7) |
| RH | ||
| 1 | 17,541,338 (76.3) | 5,436,742 (23.7) |
| 2 | 15,710,664 (72) | 6,111,477 (28) |
| 3 | 13,401,169 (71.5) | 5,352,720 (28.5) |
| 4 | 13,606,269 (75.2) | 4,497,829 (24.8) |
| 5 | 1,895,604 (67.1) | 929,937 (32.9) |
| 6 | 1,501,296 (77.5) | 436,416 (22.5) |
| 7 | 1,118,902 (63.9) | 631,314 (36.1) |
| RHΔmyr1 | ||
| 1 | 18,334,239 (78.3) | 5,080,247 (21.7) |
| 2 | 17,603,181 (72.7) | 6,625,567 (27.3) |
| 3 | 12,889,729 (64) | 7,253,952 (36) |
| 4 | 15,546,151 (71.9) | 6,078,441 (28.1) |
| 5 | 3,996,692 (63.7) | 2,276,057 (36.3) |
| 6 | 1,246,788 (68.1) | 583,369 (31.9) |
| 7 | 2,284,809 (61.8) | 1,410,089 (38.2) |
| RHΔmyr1::MYR1 | ||
| 1 | 18,645,106 (79.3) | 4,864,519 (20.7) |
| 2 | 17,179,730 (72.2) | 6,629,830 (27.8) |
| 3 | 13,579,524 (67.5) | 6,531,328 (32.5) |
| 4 | 8,443,874 (69.5) | 3,713,665 (30.5) |
| 5 | 5,650,821 (79.2) | 1,488,299 (20.8) |
| 6 | 3,624,860 (52.3) | 3,299,922 (47.7) |
| 7 | 1,409,132 (49.3) | 1,451,964 (50.7) |
| RHΔasp5 | ||
| 1 | 10,592,764 (87.4) | 1,523,876 (12.6) |
| 2 | 10,290,292 (75.1) | 3,411,275 (24.9) |
| 3 | 5,057,673 (76.8) | 1,523,876 (23.12) |
| 4 | 7,498,195 (77.9) | 2,121,971 (22.1) |
| 5 | 3,978,935 (75.5) | 1,291,803 (24.5) |
| RHΔasp5::ASP5 | ||
| 1 | 10,177,793 (65.2) | 5,433,843 (34.8) |
| 2 | 9,791,658 (81.4) | 2,235,700 (18.6) |
| 3 | 3,690,117 (63.5) | 2,123,616 (36.5) |
| 4 | 6,088,041 (86.9) | 921,158 (13.1) |
| 5 | 5,679,354 (82.4) | 1,216,067 (17.6) |
a
Shown are the numbers of raw reads mapped to human and Toxoplasma exons using CLC Genomics and the percentage of all reads mapping to these genomes.