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. 2018 Apr 3;9(2):e00451-18. doi: 10.1128/mBio.00451-18

FIG 1 .

FIG 1 

NAC form biofilms with variable characteristics. (A) Biofilm adhesion of reference strains for C. albicans (CA), C. tropicalis (CT), C. parapsilosis (CP), and C. glabrata (CG) was assessed using an XTT assay in a 96-well polystyrene plate after 1 h for adherence. The asterisks indicate statistically significant lower concentrations (P < 0.001) for C. parapsilosis and C. glabrata based upon ANOVA using the Holm-Sikak method for pairwise comparison. OD 492, optical density at 492 nm. (B) Mature biofilm formation for each of the four species was quantified in a 96-well format using an XTT endpoint after 24 h of incubation. The asterisk indicates a statistically significant lower concentration (P < 0.001) for C. glabrata based upon ANOVA using the Holm-Sikak method for pairwise comparison. (C) Mature biofilm architecture of wild-type biofilms from in vitro coverslips and the in vivo rat catheter model was assessed visually using SEM imaging after 24 h of incubation. The white arrows indicate extracellular matrix material. Bars, 20 µm.