FIG 3 .

Comparative chromatographic fractionation and NMR analysis of carbohydrates from the Candida species biofilm extracellular matrix. (A) Comparison of the 500-MHz ¹H NMR spectra of purified neutral matrix polysaccharides from C. albicans, C. tropicalis, C. parapsilosis, and C. glabrata biofilm matrix. See Table 2. (B) Comparison of C. albicans NMR spectra spin systems to each of the NAC species (C. tropicalis, C. parapsilosis, and C. glabrata, respectively) as reflected by heteronuclear single quantum coherence (HSQC) and nuclear Overhauser effect spectroscopy (NOESY) data. (C and D) Carbohydrates in the matrix of wild-type biofilms treated with tunicamycin (C) and α-mannosidase (D) were quantified by gas chromatography. Data are presented as percentages of the reference strain (Ref), with means ± standard errors (SEs) (error bars) shown. All values were significantly lower than the reference value according to ANOVA as indicated by the single asterisks.