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Expression of the pbp4 gene is higher in penicillin-resistant isolate LS4828 than in the penicillin-sensitive control strain. Cultures were grown in BHI broth to mid-log phase, and cells were harvested and frozen for RNA preparation. 16S rRNA gene expression levels were used as a reference. Error bars indicate the standard deviation of the mean of three independent experiments. *, P < 0.0001.
