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Transcriptional upregulation of pbp4 in LS4828 is due to a single base deletion in the promoter region of the gene. (A) Sequence comparison of the 200-bp region between pbp4 and the gene immediately upstream of it. There is a single base change, loss of a single A in a string of seven, upstream of a putative −35 sequence. (B) Relative expression of egfp driven by the pbp4 upstream sequence is higher from the LS4828 promoter than from the JH2-2 promoter. *, P < 0.0001.
