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Comparison of Bocillin FL binding affinities for WT JH2-2 and mutant PBP4
| PBP4 enzyme | Km (μM) | Vmaxa |
|---|---|---|
| JH2-2 | 9.0 ± 1.0 | 3.76E+07 |
| LS4828 | 57.2 ± 5.7 | 4.86E+07 |
| V223I variant | 7.7 ± 1.3 | 3.54E+07 |
| A617T variant | 60.9 ± 4.1 | 3.65E+07 |
Relative fluorescence units.
