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. 2017 Nov-Dec;28(6):362–369. doi: 10.5830/CVJA-2017-018

Fig. 1.

Fig. 1

Effect of in vitro melatonin treatment on basal and insulin-stimulated glucose uptake by cardiomyocytes from young control rats (dose response). Cardiomyocytes were isolated and incubated with melatonin and/or insulin for a period of 30 minutes. The accumulated radiolabelled 2 deoxyglucose (2DG) was measured using a scintillation counter and expressed as pmol/ mg protein/30 min. A: Effect on basal glucose uptake. Ins: insulin (1 nM), Mel: melatonin (Mel 1: 10 nM, Mel 2: 100 nM, Mel 3: 10 μM, Mel4: 50 μM), **p < 0.01 (vs basal or melatonin), n (individual preparations): n = 12 (basal), 11 (Ins), three (Mel 1), eight (Mel 2), four (Mel 3), three (Mel 4); analysed in duplicate. B: Effect on insulin-stimulated glucose uptake (fold stimulation). Ins: insulin (1 nM), Mel: melatonin (Mel 1: 10 nM, Mel2: 100 nM); *p < 0.05 (Ins vs Ins + Mel 2); **p < 0.05 (basal vs Ins or Ins + Mel 1 or 2); n = 12 (basal), 11 (Ins), five (Ins + Mel 1), six (Ins + Mel 2) individual preparations/group; analysed in duplicate.