Table I.
Substrate levels during H2 production in C. reinhardtii
| Substrate | Amount upon S Deprivation (0 h) | Amount upon Culture Sealing | Amount after 80 h of H2 Production | Changea during H2 Production |
|---|---|---|---|---|
| H2, mL | 0 | 0 | 140 | +140 |
| H2, mmol | 0 | 0 | 4.67 | +4.67 |
| CO2, mmol | 1.77 | 1.25 | 3.5 | +2.25 |
| Acetate, mmol | 15 | 7.6 | 8.2 | +0.6 (+8%) |
| Protein, mmol amino acids | 1.36 | 2.00 | 0.97 | −1.03 (−52%) |
| Starch, mmol Glc | 16 × 10−3 | 52 × 10−3 | 39 × 10−3 | −13 × 10−3 |
| (−25%) |
Values correspond to 1-L cultures with densities of 6 × 106 cells/mL at the time of sulfur deprivation (t = 0 h). H2 volume (mL) conversion to molarity (mmol) at 25°C assumed 29.97 L/mol at NREL (atmospheric pressure of 620 mm Hg at 1,600-m altitude) and 24.45 L/mol at Berkeley (atmospheric pressure of 760 mm Hg at sea level). Protein weight conversion to moles assumed an average amino acid molecular mass of 110 g/mol.
a
Change is defined as the absolute (or % in parentheses) difference between the entries of columns 4 and 3.