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Appearance of biliary cells that form hepatocytes over a time course of 21 days in organoid culture. Representative photomicrographs of biliary-specific marker HNF-1β in organoid culture tissue samples. –ve represents “no primary antibody” control. Thin arrows indicate HNF-1β+ nuclei undergoing/undergone hepatocyte to biliary transdifferentiation (HBT). Thick arrows indicate HNF-1β− nuclei that retain a hepatocytic phenotype. Original magnification: 200×, inset: 400×.
