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Genes involved with HBT in organoid culture over a time course of 21 days. mRNA levels of (A) HNF-1β, (B) Sox9, (C) Afp, and (D) Fxr as assessed by qRT-PCR and expressed as fold change relative to GAPDH. Significantly different from D3 time point: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
