Figure 1. Correlation of ALS Progression Rate With Peripheral Blood Regulatory T-Cells (Tregs).

A, Progression rate (change in Amyotrophic Lateral Sclerosis Functional Rating Scale score per month) was inversely correlated with frequency of total FoxP3⁺ Tregs, calculated as the sum of CD45RO⁺FoxP3⁺ Tregs (CD45RO⁺CD4⁺CD25^hiCD127^loFoxP3⁺) and CD45RA⁺FoxP3⁺ Tregs (CD45RA⁺CD4⁺CD25^hiCD127^loFoxP3⁺) in patients with ALS (n = 33; R = −0.41; P = .02). B, The frequency of CD45RO⁺FoxP3⁺ Tregs was inversely correlated with disease progression (R = −0.39; P = .02). C, The frequency of CD4⁺ was inversely correlated with disease progression (R = −0.36; P = .04). D, The presence of CD45RA⁺ Tregs was not correlated with disease progression (R = −0.20; P = .26). Frequencies were determined as a percentage of all leukocytes, and Pearson correlation was used on log_e-transformed cell subsets.