Figure 5. Extracellular CyPA enhances platelet-dependent thrombosis in vivo and in vitro.

Human blood was preincubated with 8H7-mAb or IgG control and stimulated with 200 nmol/L CyPA. Then the blood was perfused using a shear rate of 1000 seconds⁻¹ (A) and 1700 seconds⁻¹ (B) over a fibrillar type I collagen coated coverslip. (n ≤ 4) * means p ≤ 0.05 vs. untreated. (C) Murine blood was perfused over a CyPA/fibrillar type I collagen coated coverslip using a shear rate of 1700 seconds⁻¹ after i.v. injection of 8H7-mAb or IgG control (n ≤ 8) * means p ≤ 0.05, scale bar 20 μm. 8H7-mAb reduceed thrombus formation in vitro, and as the next step the in vivo thrombus formation in mesenteric arterioles was analyzed in a FeCl₃ model after administration of 8H7-mAb or IgG control (D/E). Representative pictures of the in vivo thrombus formation (E) and time to occlusion for individual mice (D). * means p ≤ 0.05, scale bar 50 μm.