Table 1.
Mucosal S100A12 and MPO levels in relation to histopathologic changes in the duodenum and ileum
| Histopathological findings | n | Severity groups | S100A12 (μg/L) Median (IQR) |
P valuea | MPO (ΔA/min) Median (IQR) |
P valuea |
|---|---|---|---|---|---|---|
| Duodenum | 35 | |||||
| Total histopathological severity | 35 | Insignificant (n = 16) | 33.65 (7.41-155.9) | 0.2240 | 0.9 (0.2-2.95) | 0.006 b |
| Mild (n = 15) | 53.12 (10.07-164.85) | 1.49 (0.14-4.55) | ||||
| Moderate (n = 4) | 52.22 (42-179.6) | 7.54 (0.94-17.16) | ||||
| Morphological criteria | 35 | |||||
| Villus stunting | 35 | Normal (n = 20) | 44.18 (7.41-155.9) | 0.4090 | 1.23 (0.14-4.55) | 0.1439 |
| Mild (n = 10) | 45.02 (19.51-179.6) | 1.21 (0.37-3.88) | ||||
| Moderate (n = 3) | 56.27 (42.72-164.85) | 2.16 (1.63-11.62) | ||||
| Severe (n = 2) | 26.04 (10.07-42) | 8.85 (0.54-17.16) | ||||
| Epithelial injury | 35 | Normal (n = 30) | 48.68 (7.41-164.85) | 0.9122 | 1.23 (0.14-11.62) | 0.0455 b |
| Mild (n = 3) | 28.94 (19.51-179.6) | 1.58 (0.94-2.9) | ||||
| Moderate (n = 2) | 34.38 (26.76-42) | 9.82 (2.48-17.16) | ||||
| Crypt distension | 35 | Normal (n = 29) | 42.96 (7.41-164.85) | 0.4156 | 1.29 (0.14-17.16) | 0.0694 |
| Mild (n = 5) | 67.87 (19.75-179.6) | 0.94 (0.63-1.63) | ||||
| Moderate (n = 1) | 42.72 | 11.62 | ||||
| Lacteal dilation | 35 | Normal (n = 19) | 28.94 (7.41-155.9) | 0.0889 | 1.24 (0.2-17.16) | 0.0708 |
| Mild (n = 10) | 54.7 (12.49-144.29) | 1.07 (0.14-2.48) | ||||
| Moderate (n = 6) | 53.07 (42.72-179.6) | 2.81 (0.94-11.62) | ||||
| Mucosal fibrosis | 35 | Normal (n = 31) | 42.96 (7.41-179.6) | 0.9191 | 1.36 (0.14-17.16) | 0.6993 |
| Mild (n = 4) | 63.88 (10.07-93.02) | 0.83 (0.54-3.88) | ||||
| Inflammatory criteria | 35 | |||||
| Intraepithelial lymphocytes | 35 | Normal (n = 23) | 42.96 (7.41-164.85) | 0.9381 | 1.24 (0.14-17.16) | 0.7695 |
| Mild (n = 10) | 36.44 (11.53-179.6) | 1.12 (0.37-4.55) | ||||
| Moderate (n = 2) | 53.07 (44.42-61.71) | 2.41 (1.36-3.46) | ||||
| Lamina propria LPC | 35 | Normal (n = 2) | 99.19 (42.48-155.9) | 0.1963 | 0.53 (0.2-0.87) | 0.0975 |
| Mild (n = 15) | 24.83 (7.41-164.85) | 1.24 (0.36-2.95) | ||||
| Moderate (n = 15) | 50.94 (10.07-179.6) | 1.36 (0.14-11.62) | ||||
| Severe (n = 3) | 61.71 (42-93.02) | 3.46 (0.77-17.16) | ||||
| Lamina propria eosinophils | 35 | Normal (n = 22) | 58.99 (10.07-179.6) | 0.0516 | 0.92 (0.14-3.46) | 0.0004 b |
| Mild (n = 5) | 19.75 (7.41-42.96) | 0.91 (0.64-2.95) | ||||
| Moderate (n = 8) | 43.33 (26.76-78.03) | 3.39 (1.22-17.16) | ||||
| Lamina propria neutrophils | 35 | Normal (n = 20) Mild (n = 7) Moderate (n = 6) Severe (n = 2) |
42.72 (7.41-102.46) 61.1 (19.51-155.9) 47.92 (24.83-179.6) 113.28 (61.71-164.85) |
0.1542 | 1.07 (0.36-17.16) 1.49 (0.2-3.88) 1.4 (0.14-11.62) 2.81 (2.16-3.46) |
0.7326 |
| Lamina propria macrophage | 35 | Normal (n = 32) | 42.84 (7.41-164.85) | 0.0439 b | 1.39 (0.14-17.16) | 0.1786 |
| Mild (n = 3) | 155.9 (44.2-179.6) | 0.94 (0.2-1.36) | ||||
| Ileum | 12 | |||||
| Total histopathological severity | 12 | Insignificant (n = 8) | 106.93 (9.59-317.21) | 0.9816 | 1.91 (0.34-2.83) | 0.3260 |
| Mild (n = 4) | 125.92 (16.36-151.79) | 2.3 (0.79-7.01) | ||||
| Morphological criteria | 12 | |||||
| Villus stunting | 12 | Normal (n = 7) | 120.84 (9.59-317.21) | 0.7443 | 2.04 (0.34-2.83) | 0.3447 |
| Mild (n = 5) | 116.24 (16.36-151.79) | 1.77 (0.69-7.01) | ||||
| Epithelial injury | 12 | Normal (n = 10) | 118.54 (9.59-317.21) | 0.4956 | 1.91 (0.34-3.23) | 0.2387 |
| Moderate (n = 2) | 84.07 (16.36-151.79) | 4.18 (1.36-7.01) | ||||
| Crypt distension | 12 | Normal (n = 11) | 116.24 (9.59-317.21) | 0.6913 | 2.04 (0.34-7.01) | 0.4692 |
| Moderate (n = 1) | 120.84 | 0.79 | ||||
| Lacteal dilation | 12 | Normal (n = 8) | 106.93 (9.59-317.21) | 0.9881 | 1.56 (0.34-2.83) | 0.2034 |
| Mild (n = 4) | 125.92 (16.36-162.43) | 2.63 (0.79-7.01) | ||||
| Mucosal fibrosis | 12 | Normal (n = 12) | 118.54 (9.59-317.21) | – | 1.91 (0.34-7.01) | – |
| Inflammatory criteria | 12 | |||||
| Intraepithelial lymphocytes | 12 | Normal (n = 6) | 109.23 (9.59-162.43) | 0.6809 | 1.42 (0.34-2.81) | 0.1787 |
| Mild (n = 6) | 123.62 (16.36-317.21) | 2.3 (0.69-7.01) | ||||
| Lamina propria LPC | 12 | Normal (n = 4) | 118.78 (47.08-162.43) | 0.5561 | 2.12 (0.44-2.81) | 0.9999 |
| Mild (n = 4) | 68.6 (9.59-317.21) | 1.81 (0.34-7.01) | ||||
| Moderate (n = 4) | 123.62 (47.32-151.79) | 1.56 (0.69-3.23) | ||||
| Lamina propria eosinophils | 12 | Normal (n = 9) | 130.99 (9.59-317.21) | 0.2514 | 1.77 (0.34-3.23) | 0.2019 |
| Mild (n = 2) | 72.35 (47.08-97.62) | 1.63 (0.44-2.81) | ||||
| Moderate (n = 1) | 16.36 | 7.01 | ||||
| Lamina propria neutrophils | 12 | Normal (n = 9) | 116.24 (9.59-317.21) | 0.7430 | 2.04 (0.34-3.23) | 0.5989 |
| Mild (n = 3) | 120.84 (16.36-151.79) | 1.36 (0.79-7.01) | ||||
| Lamina propria macrophage | 12 | Normal (n = 12) | 118.54 (9.59-317.21) | – | 1.91 (0.34-7.01) | – |
Association between mucosal S100A12 concentrations and MPO activities with severity of histopathological findings in the duodenum and ileum of dogs with CE. Statistical differences were calculated based on the log-transformed values of S100A12 and MPO. Untransformed values are reported as median (IQR)
n sample size, IQR interquartile range, LPC lymphocytes/plasma cells
aANOVA test after logarithmic transformation of original values
bstatistically significant association