Figure 2.

HFD impairs SIRT3-MnSOD and increases hippocampal oxidative stress. (a) The acetylation level of mitochondria protein was examined by immunoblots. (b) The acetylation of MnSOD was examined by coimmunoprecipitation. (c) MnSOD activity. (d) ROS levels in hippocampus. (e) mda levels in hippocampus. (f) The combination of SIRT3 and MnSOD was performed in vitro. (g) Cell death of hippocampal neurons with H₂O₂ treatment in vitro. ^∗P < 0.05 versus WT; ^#P < 0.05 versus HFD. Values are means ± SEM, n = 6 independent experiments.