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. 2018 Apr 5;9:29. doi: 10.1186/s40104-018-0245-1

Table 1.

Amplification and sequencing primers for the 16S rRNA and gyrB genes

Gene Primer Primer sequence (5′→3′)
16S rRNA BSF8/20 (Forward) AGAGTTTGATCCTGGCTCAG
BSR534/18 (Reverse) ATTACCGCGGCTGCTGGC
gyrB UP-1 (Forward) GAAGTCATCATGACCGTTCTGCA(TC)GC(TCAG)GG(TCAG)GG(TCAG)AA(AG)TT(TC)GAGAAGTCATCATGACCGTTCTGCAYGCNGGNGGNAARTTYGA
UP-2r (Reverse) AGCAGGGTACGGATGTGCGAGCC(AG)TC(TCAG)AC(AG)TC(TCAG)GC(AG)TC(TCAG)GTCATAGCAGGGTACGGATGTGCGAGCCRTCNACRTCNGCRTCNGTCAT
UP1S (Forward) GAAGTCATCATGACCGTTCTGCA
UP2SR (Reverse) AGCAGGGTACGGATGTGCGAGCC