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N-terminal truncated ACA2 pumps Ca2+ with high velocity and affinity. Membranes were isolated from yeast expressing ACA2-2. The initial rate of vanadate-sensitive Ca2+ uptake (30 s) was determined as a function of external [Ca2+] in the presence of bafilomycin and CCCP. The Km for Ca2+ of 0.2 μm was estimated using a Lineweaver-Burk plot. The average of duplicates from one of three experiments is shown.
