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. 2018 Apr 4;11:27. doi: 10.1186/s13048-018-0399-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The effect of paclitaxel resistance on key regulators of the mitotic spindle checkpoint. Immunoblot analysis of cell cycle proteins BubR1, cyclin B1, CENPE and CENPF isolated from native and resistant cell lines. Individual lanes contained 20-50 μg of total protein; each gel was normalized. The proteins were resolved on 4-20% Mini Protean TGX Precast Gels, transferred to nitrocellulose membranes and probed using antibodies specific for BubR1, cyclin B1, CENPE and CENPF proteins. β-actin served as a loading control. The immunoblots represent a single experiment; 3 independent experiments were conducted