. 2018 Apr 5;17:55. doi: 10.1186/s12934-018-0902-2

Table 1.

Characteristics and purification strategies of recombinant proteins

Antigen^a	Accession number	Target sequence	Cysteine (no)	Disorder tendency^b	Expression level		Capturing		Polishing		Mass (Da)
Antigen^a	Accession number	Target sequence	Cysteine (no)	Disorder tendency^b	No carrier	With carrier	IMAC	Heat	IEC	Yield (mg/l)^c	Calculated	Observed^d
GPI-anchored
MSP1_19k	PFI1475W	N1607–N1702	12	0	High		X		A	30	12.2	15
MSP2^3D7	PFB0300C	A111–G238	0	100	High		X		A	20	13.7	40
MSP2^FC27		E143–G230	0	94	High		X		A	20	9.6	25
Pf12	PFF0615C	K155–S323	6	0	Low	Med		X	C	10	20	20
Pf38	PFE0395C	K139–I327	6	0	Low	Med		X	C	10	23	25
Peripherally associated
nMSP3^3D7	PF10_0345	N21–L182	1	25	High		X		A	40	17.6	27
cMSP3^3D7	PF10_0345	K183–H354	0	0	High		X		A	18	20	30
nMSP3^K1	PFU08851	N21–L208	0	48	High		X		A	35	21	37
MSP6	PF10_346	Y17–N160	0	70	Med		X		A	10	17.5	23
MSP3.3	PF10_0347	Q23–T227	0	48	Med		X		A	7	22.3	37
MSP3.7	PF10_0352	K27–R213	0	100	Med		X		A	7	20.9	37
GLURP-R0	AAA50613	T27–A500	0	99	High		X		A	75	54.3	90
GLURP-R2	AAA50613	V705–1177	0	78	High		X		A	35	53	110
SERA5	PF02_0072	K17–192/226–S382	8	40	Low	High		X	C	17	35.5	38
Pf41	PFD0240C	S231–S378	6	0	Low	Med		X	C	7	18	18
MSPDBL1	PF10_0348	K143–D443	13	0	Low	Med		X	C	8	37	37
MSPDBL2	PF10_0355	K161–L457	12	0	Low	High		X	C	15	36.5	35
MSPDBL1 (Leucine)	PF10_0348	D508–K697	0	69	Med		X		A	7	22	35
Micronemal/microneme
EBA140RIII–V	MAL13P1.60	E746–T1045	0	93	High		X		A	16	32.9	40
EBA140 RII	MAL13P1.60	Q146–K710	23	0	Low	Low		X	C	1,5	67.3	85
GAMA	PF08_0008	N25–H337	7	10	Low	Med		X	A	5	36.8	45
RIPR	PFC1045C	K279–D995	75	0	Low	Low		X	A	1	82.3	110
SUB2	PF11_0381	K14–N681	2	29	Low	Med		X	A	5	78.3	90
Rhoptry
PfRh2b	MAL13P1.176	L2790–K3184	0	91	Med		X		A	8	44.7	120
PfRh2a	PF13_0198	H2874–S3060	0	26	High		X		A	13	22	25
PfRh4.2	PFD1150C	L1275–I1451	0	11	Low	Med	X		C	2	21.5	21
PfRh2-2030	PF13_0198	E2030–Q2528	1	0	Med		X		A	8	58	58
RAMA	MAL7P1.208	I42–N786	0	100	Low	Med	X		A	7	90.6	120
RALP-1	MAL7P1.119	N396–P634	0	47	Low	Med	X		A	10	27	37
RON2	PF14_0495	I775–K958	0	49	Med		X		A	10	24	25
RON4	PF11_0168	K25–L709	0	100	Low	Med	X		A	9	76	150

A anion exchange (HiTrap Q HP); C cation exchange (Hitrap SP HP)

^aGene originate from 3D7 unless otherwise stated

^bDisorder prediction score was calculated by using IUPred [41]

^cThe levels of expressed protein was determined by BCA kit after final step purification. Expression levels are grouped into “low” (0.1–2 mg/l), “medium” (2–10 mg/l), and “high” (> 10 mg/l)

^dBy SDS-PAGE