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. 2018 Apr 4;11:226. doi: 10.1186/s13071-018-2818-4

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© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — a SDS-PAGE analysis of the soluble proteins of E. maxima sporozoites. Twenty micrograms of protein samples were subjected to a 12% gel. Lane M: standard protein molecular marker; Lane 1: the soluble proteins of E. maxima sporozoites. b Western blot analysis of the soluble proteins of E. maxima sporozoites. Proteins are recognized by sera from rats as primary antibody. Lane M: standard protein molecular marker; Lane 2: sera from rat immunized with the soluble proteins of E. maxima sporozoites; Lane 3: pre-immune rat sera