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. 2018 Apr 4;11:226. doi: 10.1186/s13071-018-2818-4

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© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Western blot analysis of proteins of chicken jejunal epithelial cells co-cultured with the soluble proteins of E. maxima sporozoites. Proteins are recognized by sera from rats as primary antibody. Lane M: standard protein marker; Lane 1: lysis of chicken jejunal epithelial cells cultured with E. maxima sporozoites soluble proteins detected by sera from rats immunized with soluble proteins of E. maxima sporozoites; Lane 2: lysis of chicken jejunal epithelial cells cultured with PBS buffer detected by sera from rats immunized with soluble proteins of E. maxima sporozoites; Lane 3: lysis of chicken jejunal epithelial cells cultured with E. maxima sporozoites soluble proteins detected by pre-immune rat sera