Table 1.
Proteins of E. maxima that bound to chicken jejunal epithelial cells identified by shotgun LC-MS/MS with more than two unique peptide counts
| Protein description | Accession number | Unique pep count MH+ | Pep count (sequence) | Cover percent (%) | Theor MW (kDa) | Theor pI | MASCOT score |
|---|---|---|---|---|---|---|---|
| Lactate dehydrogenase | gi|557194155 | 4 | 4 | 16.97 | 35.94 | 6.60 | 60.84 |
| 14-3-3 protein | gi|557193108 | 6 | 6 | 23.43 | 32.55 | 4.80 | 63.59 |
| Elongation factor 1-alpha | gi|557198794 | 3 | 4 | 6.44 | 49.36 | 9.16 | 63.00 |
| Elongation factor 2 | gi|557157066 | 3 | 3 | 3.37 | 93.28 | 5.99 | 49.12 |
| Fructose-bisphosphate aldolase | gi|557193254 | 3 | 3 | 13.13 | 38.77 | 8.33 | 62.10 |
| ATP synthase beta chain | gi|557185041 | 3 | 3 | 4.80 | 72.32 | 6.22 | 47.06 |
| 18 kda cyclophilin | gi|557198725 | 3 | 3 | 23.28 | 20.35 | 6.97 | 54.77 |
| Hypothetical protein, conserved | gi|557206977 | 3 | 3 | 11.67 | 32.25 | 4.58 | 53.47 |
| Heat-shock protein 60 | gi|557243650 | 3 | 3 | 8.73 | 59.93 | 5.86 | 53.46 |
| B-block-binding subunit of tfiiic protein | gi|557157037 | 2 | 9 | 0.46 | 34.63 | 6.76 | 44.91 |
| Polyubiquitin | gi|557209304 | 2 | 5 | 11.54 | 17.46 | 8.01 | 39.48 |
| RAS small GTpase | gi|557167708 | 2 | 3 | 8.33 | 22.94 | 7.64 | 71.94 |
| Heat-shock protein, related | gi|557184708 | 5 | 5 | 7.65 | 71.41 | 5.16 | 80.62 |
| Microneme protein 7 | gi|343094702 | 2 | 2 | 15.12 | 18.24 | 4.62 | 57.25 |
| Tubulin beta chain | gi|343480759 | 2 | 2 | 6.01 | 49.92 | 4.78 | 76.11 |
| ATP synthase alpha | gi|557156948 | 2 | 2 | 6.90 | 21.68 | 7.63 | 43.05 |
| SWI/SNF-related matrix-associated actin-dependent regulator of chromatin | gi|557158395 | 2 | 2 | 1.51 | 11.91 | 5.64 | 41.06 |
| ADP ribosylation factor 1 | gi|557167614 | 2 | 2 | 11.40 | 20.80 | 6.10 | 56.12 |
| Protein emb isoform, related | gi|557184804 | 2 | 2 | 0.61 | 23.58 | 5.80 | 35.63 |
| Hypothetical protein, conserved | gi|557168315 | 2 | 2 | 0.38 | 38.08 | 6.14 | 29.12 |
| Hypothetical protein, conserved | gi|557210462 | 2 | 2 | 0.26 | 63.97 | 6.65 | 34.58 |
| 60s ribosomal protein L3 | gi|557210678 | 2 | 2 | 4.90 | 44.11 | 10.41 | 60.01 |
| Heat-shock protein 90 | gi|557188011 | 2 | 2 | 2.94 | 82.69 | 5.08 | 76.54 |
| Microneme protein 2 | gi|334851460 | 5 | 5 | 14.64 | 30.60 | 4.90 | 51.92 |
| Heat-shock protein 70 | gi|557232143 | 2 | 2 | 3.25 | 56.17 | 8.69 | 46.71 |
| Triosephosphate isomerase | gi|557232104 | 2 | 2 | 10.76 | 27.36 | 6.00 | 38.99 |
| Tubulin alpha chain | gi|557210668 | 2 | 2 | 4.98 | 46.69 | 4.90 | 55.07 |
| Microneme protein 3, partial | gi|557237363 | 2 | 2 | 4.58 | 44.33 | 4.65 | 51.70 |
| Coiled-coil domain-containing protein, related | gi|557238523 | 2 | 2 | 0.45 | 26.90 | 4.52 | 26.72 |
| Actin | gi|557207008 | 4 | 15 | 12.77 | 42.01 | 5.00 | 49.73 |
| Histone | gi|19880141 | 4 | 9 | 20.59 | 15.39 | 11.29 | 35.08 |
| 82 kda gametocyte, related | gi|38565038 | 4 | 5 | 9.73 | 66.09 | 5.16 | 101.05 |
| 56 kda gametocyte, related | gi|557167757 | 4 | 5 | 9.03 | 53.30 | 5.00 | 78.91 |
| Enolase 2 | gi|557157196 | 4 | 4 | 9.37 | 49.88 | 5.92 | 42.82 |
| Glyceraldehyde-3-phosphate dehydrogenase | gi|557168185 | 4 | 4 | 11.21 | 36.26 | 7.58 | 74.56 |