Fig. 6.

HCC cell-derived acetoacetate suppresses macrophage recruitment by inhibiting MIF activity. a The tautomerase activity of secreted MIF from 7402 cells with B3GALNT2 level changes. b Secreted levels of MIF in the culture media of B3GALNT2-overexpressed 7402 cells determined by ELISA. c NAPQI attenuated the macrophage recruitment ability of B3GALNT2-overexpressed 7402 cells in transwell assays. d Knockdown efficiency of MIF in the 7402 cell line as determined by qPCR and ELISA. e MIF knockdown attenuated the macrophage recruitment ability of B3GALNT2-overexpressed 7402 cells in transwell assays. f MIF knockdown attenuated the macrophage recruitment ability of BDH2-knockdown 7402 cells in transwell assays. g Schematic describing how B3GALNT2 functions in HCC