Figure 4.

Different effects of high CO₂/low O₂ atmospheres on border cell separation from pea (A) and alfalfa (B). Pea and alfalfa seeds were germinated and grown under ambient atmospheric conditions in a controlled-temperature incubator and seedlings with established roots (25 mm for pea and 20 mm for alfalfa) and a whole set of border cells were transferred into indicated test atmospheres. Seedlings were collected and border cell numbers were counted from each treatment before (0) and 1, 2, and 3 d after the treatment. There were three replicates in each treatment and each sample was the average of five seedlings. The whole test was performed twice. Values are the means of six replicates and bars represent se.