Skip to main content
. Author manuscript; available in PMC: 2018 Apr 5.
Published in final edited form as: Nature. 2017 May 17;545(7655):482–486. doi: 10.1038/nature22365

Extended Data Figure 9. Sequence alignment of NS1 sequences in African ZIKV isolates, and NS1 secretability in African ZIKV-infected Vero cells.

Extended Data Figure 9

a, Sequence alignment of NS1 sequences in African ZIKV isolates.

b-d, NS1 secretability in African ZIKV-infected Vero cells. Vero cells were infected at an M.O.I. of 0.01 with the GZ01, FSS13025 and MR766 strains. Supernatants from the infected Vero cells were collected from 0 to 4 days post-infection. b, ZIKV titer was determined by a plaque assay. c, The amount of ZIKV NS1 protein was determined by ELISA. d, NS1 quantity normalized against average viral titer (ng per pfu). The data were pooled from 3 independent biological replicates.

The values in the graph represent the mean ± s.e.m. (b-d).