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Refolding of Hsp18.1-bound Luc by the DnaK system. Luc (1 μm) was heated in the presence of 1 μm Hsp18.1 (Hsp18.1/Luc, ●), 2 μm DnaK/0.4 μm DnaJ/0.8 μm GrpE (KJE + Luc, ▴) or 0.21 mg/mL bovine IgG (IgG + Luc, ○) at 42°C for either 8 min in the absence of ATP (A) or for 30 min in the presence of ATP (B). Samples containing 25 nm Luc were then added to 1.5 μm DnaK/0.3 μm DnaJ/0.6 μm GrpE and ATP and allowed to refold at 30°C.
