Figure 3.

Hsp18.1 maintains higher Luc solubility than Hsp70 systems. Luc (1 μm) in the presence of ATP and 0.7 μm Hsp18.1, 2 μm Hsc70/0.15 μm Hdj1/0.15 μm Ydj1 (70J), 2 μm DnaK/0.4 μm DnaJ/0.8 μm GrpE (KJE), or 0.21 mg/mL bovine IgG was maintained at 42°C for 30 min. Samples were centrifuged for 15 min at 16,250g, separated into supernatant (S) or pellet (P) fractions, and then analyzed by SDS-PAGE and Coomassie Blue staining. The Hsp18.1 bands appear narrow because they are compressed at the buffer front.