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. Author manuscript; available in PMC: 2018 Apr 5.
Published in final edited form as: Bioessays. 2018 Jan 23;40(3):10.1002/bies.201700176. doi: 10.1002/bies.201700176

Figure 3.

Figure 3

Stabilizing kinetochore-microtubule interactions and silencing the spindle checkpoint by the Ska complex. In prophase/prometaphase when kinetochores are poorly attached by microtubules, the kinetochore-localized Mps1 phosphorylates the MELT domains in Knl1 to recruit downstream effectors, thus activating the spindle checkpoint. At metaphase, microtubule attachments to kinetochores displace Mps1 from kinetochores and the kinetochore-microtubule attachments are stabilized by the Ska complex recruited by the Ndc80 N-terminus. At the same time, multiple copies of phospho (p)-MELT domains distributed along a wide range of amino-acid sequences in Knl1 are dephosphorylated by both the Ndc80-loop pool of Ska-PP1 and the Knl1-based PP1. The Ndc80-loop pool might provide extra dephosphorylating strength to more efficiently remove the phosphorylation from the phospho-MELT domains.