Fig. 10. (a) Flash-induced absorption changes on a ms time scale for 21.3 μM W388F mutant MmCPDII at selected wavelengths (for all measured signals, see Fig. S4†). The beginning of some of the signals is distorted by a fluorescence artifact. (b) Signal amplitudes at t = 1 ms (i.e., after the end of the fluorescence artifact) are compared with the difference spectrum for the formation of FAD˙^– + Tyr˙. All signals exhibit a monoexponential decay with a time constant of 2.7 ms. Sample was excited at 470 nm by a 5 ns pulse of E ∼ 10 mJ per cm². The signals are averages of three single-flash experiments spaced by ∼1 minute.