Fig. 11. Flash-induced absorption changes at 562 nm showing TrpH˙+ deprotonation in 130 μM E387Q mutant MmCPDII in comparison with the WT protein from Fig. 5 (normalized to the amplitude of E387Q). Fitting by a monoexponential decay function gives lifetimes of ∼350 ps for WT and ∼500 ps for E387Q. Both samples were excited at 355 nm by a 100 ps pulse of E ∼ 2.5 mJ per cm2; the traces are averages of 64 (E387Q) or 512 (WT) signals recorded with a repetition rate of 1 Hz.
