Fig. 3. (a) Flash-induced absorption changes on a ns/μs timescale for 40 μM WT MmCPDII at seven characteristic wavelengths (see Fig. S1† for signals at all measured wavelengths). (b) Signal amplitudes at t → 0 compared to difference spectra for the formation of FAD˙^– + Trp˙ (black solid line) and of FAD˙^– + TrpH˙⁺ (red dashed line). The sample was excited at 470 nm by a 5 ns pulse of an energy E ∼ 5 mJ per cm². Individual traces are averages of four single flash signals spaced by ∼1 minute.