Fig. 8. (a) Flash-induced absorption changes on a ns/μs timescale for 38.5 μM W388F mutant MmCPDII at seven characteristic wavelengths. (b) Signal amplitudes at t = 1.5 μs are compared to the difference spectrum for the formation of FAD˙– + Tyr˙. The sample was excited at 470 nm by a 5 ns pulse of E ∼ 5 mJ per cm2. The traces are averages of 8, 16 or 32 signals recorded with a repetition rate of 1 Hz.
