Table 1.

Estimation of the percentage of BrdU- and Sca-1 positive cells in stromal-vascular fraction of epididymal and inguinal fat tissues and bone marrow in respect to animals age and diets.

		EPIDYDYMAL FAT DEPOT				INGUINAL FAT DEPOT				BONE MARROW				% of cells stained by BrdU+Sca1 vs BrdU alone
age	diet	% of BrdU		% of Sca1+BrdU		% of BrdU		% of Sca1+BrdU		% of BrdU		% of Sca1+Brdu		eWAT	iWAT	BM
4 weeks	LFD	3.04	(±1.28)	2.28	(±1.05)	0.87	(±0.36)	0.71	(±0.38)	0.07	(±0.02)	0.042	(±0.02)	75	81.6	60
8 weeks	LFD	1.46	(±0.59)	1.3	(±0.57)	0.62	(±0.34)	0.52	(±0.28)	0.04	(±0.01)	0.025	(±0.01)	89.1	83.8	62.5
8 weeks	HFD	1.53	(±0.51)	1.3	(±0.37)	0.66	(±0.26)	0.58	(±0.23)	0.02	(±0.01)	0.02	(±0.01)	84.9	87.9	100
16 weeks	LFD	1.22	(±0.39)	1.15	(±0.36)	1.09	(±0.26)	0.77	(±0.18)	0.02	(±0.01)	0.01	(±0.01)	94.3	91.7	50
16 weeks	HFD	0.69	(±0.60)	0.67	(±0.60)	* ###1.93	(±0.15)	* ###1.7	(±0.17)	0.01	(±0.01)	0	(±0)	97.1	94.4	0
30 weeks	LFD	1.19	(±0.21)	1.16	(±0.20)	1.06	(±0.14)	0.98	(±0.12)	0.04	(±0.01)	0.025	(±0.01)	97.5	92.5	71.4
30 weeks	HFD	** #0.35	(±0.07)	** #0.34	(±0.07)	**0.38	(±0.11)	**0.37	(±0.11)	0.03	(±0.01)	0.02	(±0.01)	97.1	97.4	74.1

Percentage of cells from SVF of epididymal (eWAT), inguinal (iWAT) and bone marrow (BM) that stained positive for: BrdU; BrdU+Sca-1; and BrdU+Sca-1 vs BrdU alone based on flow cytometric analysis. The data represents the mean of four experiments ±SD; each experiment consisted of a pool of cells from two animals;

^*p<0.05 LFD vs HFD at 16 weeks of age

^**p<0.01 LFD vs HFD at 30 weeks of age,

^# p<0.05 4 vs 30 weeks of age,

^### p<0.001 8 vs 16 weeks of age on HFD.