(A) Representative immunolabeling of paraffin-embedded heart sections stained for macrophages (CD68 specific marker for macrophages), myeloperoxidase, and STIM1 in C57/Bl6 (C57), C57 + I/R injury, and Stim1SMC−/− + I/R injury (n=4–6). The brown dark brown spots indicate immunopositive reaction to CD68, myeloperoxidase, and STIM1 antibodies. (B) Real-Time PCR for CCL2, IL-6, Interferon gamma (INFγ), and Tumor necrosis factor alpha (TNFα). *P<0.05 for C57 + I/R injury vs. Stim1SMC−/− + I/R injury and C57 without I/R injury.