Figure 1.

Inducible inactivation of Wnt16 in young adult male mice reduces cortical bone thickness. Fourteen-week-old male Cre-Wnt16^flox/flox (n = 7) and littermate Wnt16^flox/flox (n = 6) control mice treated for four consecutive days with either low-dose (0.25 mg/mouse/day) or high-dose (1 mg/mouse/day) tamoxifen at the age of ten weeks. (A) Expression level of Wnt16 mRNA in cortical bone of femur, gonadal fat and liver. (B) Cortical bone thickness of femur as analyzed using μCT. ND, not detectable. Values are given as mean ± s.e.m. (Wnt16^flox/flox low-dose, n = 6, high-dose n = 7; Cre-Wnt16^flox/flox low and high dose, n = 7). *P < 0.05, **P < 0.01, Student’s t test, Cre-Wnt16^flox/flox vs Wnt16^flox/flox control mice. (C) Correlation between Wnt16 mRNA levels in cortical bone of femur and cortical bone thickness in the femur diaphysis. Pearson’s correlation coefficient is given.