Figure 2.

Inducible inactivation of Wnt16 in older female mice reduces cortical bone thickness. 51-week-old female Cre-Wnt16^flox/flox and littermate Wnt16^flox/flox control mice treated with tamoxifen (1 mg/mouse/day) for four consecutive days at 47 weeks of age. (A) Wnt16 mRNA levels in cortical diaphyseal bone of femur. (B) Cortical thickness of the mid-diaphysis of femur as analyzed using µCT and (C) maximal (max.) load at failure determined by three-point bending of humerus. (D) mRNA levels of CatK, Opg and Rankl in cortical diaphyseal bone of femur. Values are given as mean ± s.e.m. (Wnt16^flox/flox n = 11; Cre-Wnt16^flox/flox n = 9). **P < 0.01, Student’s t test, Cre-Wnt16^flox/flox vs Wnt16^flox/flox control mice.