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. 2017 Oct 10;27(1):199–210. doi: 10.1093/hmg/ddx375

Figure 4.

Figure 4.

The BP-associated variant rs1173747 affects chromatin status and nuclear protein binding. In this figure, ‘E’ indicates BP-elevating allele, ‘L’ denotes BP-lowering allele. (A) Formaldehyde-assisted isolation of regulatory elements followed by allelic imbalance assays by Sanger sequencing. Representative images showing different peak heights of the E and L alleles of rs1173747 in chromatograms derived from reference (Ref.) DNA and FAIRE DNA. Mean±SEM of n=6 heterozygous samples, *P <0.05. (B) Representative result of electrophoretic mobility shift assay with the use of biotin-labelled double-stranded oligonucleotide probes and unlabelled competitors of the E and L alleles of rs1173747 and nuclear protein extracts from VSMCs. (C) Relative activity of the rs1173747 E and L alleles, respectively, in driving firefly luciferase reporter gene expression in transfected VSMCs, standardized for transfection efficiency. Mean±SEM, **P <0.01, ***P <0.001.