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. 2017 Oct 10;27(1):199–210. doi: 10.1093/hmg/ddx375

Figure 5.

Figure 5.

The BP-elevating variant at NPR3 locus is associated with increased human VSMCs proliferation. VSMCs in the absence and presence of C-type natriuretic peptide (CNP; 100 nmol/l) and the NPR-C specific agonist (cANF4–23; 100 nmol/l) for 24 h were analysed for proliferation rate. Cell proliferation over the 24-h period was calculated as (OD450 at 24 h−OD450 at baseline)/OD450 at baseline. ‘E’ indicates the BP-elevating allele, ‘L’ denotes the BP-lowering allele. (A) Differences in proliferation between untreated cells of different genotypes. Representative images of DAPI stained cells of the three different genotypes at baseline and 24 h. Scale bars=200µm. Mean±SEM, *P <0.05. (B) Differences in proliferation between CNP or cANF4–23 treated cells of different genotypes, and (C) Inhibitory effects of CNP and cANF4–23 on proliferation of cells of different genotypes, calculated by (OD450 at 24 h of untreated cells−OD450 at 24 h of CNP-treated or cANF4–23-treated cells)/OD450 at 24 h of untreated cells. Mean±SEM, *P <0.05.